Histopathological investigation of the effect of levofloxacin and cephalexin derivative antibiotics on bone healing in experimentally induced fractures in rat tibias
Abstract
This study compared the effects of systemic Levofloxacin and Cephalexin on bone healing in a rat tibial fracture model. The subjects were divided into six groups (n=7 each): healthy control, fracture control, healthy Levofloxacin, healthy Cephalexin, fractured Levofloxacin, and fractured Cephalexin. In total, 42 Sprague–Dawley rats were used. In the fractured groups, a transverse fracture was created in the right tibial diaphysis, and the bone fragments were stabilized with Kirschner wire. No surgical intervention was performed in the healthy control and healthy antibiotic groups. The antibiotic groups received systemic Levofloxacin (25 mg/kg) or Cephalexin (20 mg/kg) three times a week for 4 weeks, starting on the same day as the fractured subjects. At the end of the experimental process, all animals were euthanised. After the decalcification process, the obtained samples were evaluated histopathologically, and bone filling ratio percentages were analyzed using histomorphometric methods. In the statistical analysis, differences between groups were examined using one- way analysis of variance. In cases where the variances were not homogeneous, pairwise comparisons were performed using the Games–Howell post-hoc test. Bone formation ratios (%) showed significant differences between the groups (P < 0.05). In this study, bone formation rates were found to be 90.71 in the healthy control group, 67.86 in the fracture control group, 61 in the fracture cefelaxin group, 51.14 in the…
Read the paper
Related studies
- Ferulic acid regulates the AKT/GSK-3β/CRMP-2 signaling pathway in a middle cerebral artery occlusion animal model
- Novel Pharmacokinetic/Pharmacodynamic Parameters Quantify the Exposure–Effect Relationship of Levofloxacin against Fluoroquinolone-Resistant Escherichia coli
- Photochemical behavior of levofloxacin
- Correlation Between Dissolution Profiles of Salt-Form Drugs in Biorelevant Bicarbonate Buffer and Oral Drug Absorption: Importance of Dose/ Fluid Volume Ratio
- Evaluation of the synergistic potential and mechanisms of action for de novo designed cationic antimicrobial peptides
- Synthesis of [11C]levofloxacin
Go deeper in Evidence Hub
Evidence Hub adds a study data sheet (design, sample size, evidence level, key numbers), an AI summary and a chat grounded in the paper's text.