Evaluation of Levofloxacin Release Characteristics from a Human Foldable Capsular Vitreous Body In Vitro
Abstract
Abstract Purpose: We have manufactured a novel human foldable capsular vitreous body (FCVB). In this article, we determine whether the human FCVB releases levofloxacin in vitro and evaluate the release characteristics. Methods: Levofloxacin at dosages of 100, 250, and 500 μg/mL in 4.0 mL H2O was injected into the novel human FCVB; then, the capsules were immersed in cups of modified Franz diffusion cells. Two hundred microliters of liquid was aspirated at intervals of 10, 20, 40, 60, 120, 180, 240, 300, and 360 min. The levofloxacin concentrations in the cups were detected using the liquid chromatographic-tandem mass spectrometry (HPLC-MS/MS) method. Five hundred μg/mL levofloxacin liquor at volumes of 4.0, 5.0, and 6.0 mL was injected into the novel human FCVB, and the release characteristics were detected for each. The capsules of the FCVB were observed under scanning electron microscopy before and after the release study. Results: The novel human FCVB released levofloxacin in a time-dependent manner under 100, 250, and 500 μg/mL dosages from 10 to 360 min. Particularly in the 250 μg/mL levofloxacin group, the concentration (Q) had a good linear relationship with time (t1/2), Q=1.3381t1/2+10.2818 (r=0.9954, Higuchi equation). The human FCVB released levofloxacin in a dose-dependent manner under 100, 250, and 500 μg/mL dosages from 10 to 360 min in the total tendency, but the 500 μg/mL group released less levofloxacin than the 250 group at 300 and 360 min. The human FCVB…
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