Effect of baicalein combined with levofloxacin on Staphylococcus aureusbiofilm in vitro
Abstract
OBJECTIVE To investigate the effect of baicalein combined with levofloxacin(LFX)on the Staphylococcus aureus biofilm(BF) so as to guide the clinical medication. METHODS The model of S.aureus biofilm was built in vitro;then the models were divided into six groups: the control group,baicalein group,clarithromycin(CLARY) group,LFX group,CLARY +LFX group,and the baicalein+LFX group;the MIC and MBC of test drugs were measured by the double dilution method;the bacterial colony counts in BF were recorded by serial dilution method;the shapes of S.aureus BF were observed through the confocal laser scanning microscope(CLSM).RESULTS A total of 17 546 strains of Early(3d) and mature(7d) S.aureus BF models were established successfully.The early bacterial colony count results showed that the difference in the bacterial colony count between the early S.aureus BF,mature S.aureus BF,and the control group was not statistically significant;the bacterial colony count was(7.19±0.12)log10 CFU/ml in the early baicalein+LFX group,(7.25±0.13)log10 CFU/ml in the early CLARY+LFX group,as compared with(7.88±0.10)log10 CFU/ml of the control group and(7.81±0.07)log10 CFU/ml of the LFX group,the bacterial colony count decreased significantly(P0.05);the bacterial colony count was(7.29±0.09)log10 CFU/ml in the mature Baicalein+LFX group,(7.36±0.11)log10 CFU/m in the mature CLARY+LFX group,as compared with(7.89±0.12)log10 CFU/ml of the control group or(7.87±0.08)log10 CFU/ml of the LFX group,the bacterial…
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