The application of arbutin in elimination resistance gramm-negative multidrug resistance bacteria of Pseudomonas aeruginosa and Enterobacter cloacea
Abstract
The basic therapy against microbial infections is the application of antibiotics. However, overuse of antibiotics has become the major factor for the emergence and dissemination of multidrug-resistant strains.
The aim. The purpose of our work was to investigate the in vitro and in silico elimination resistance of antibiotics (сlarithromycin, azithromycin, gentamycin, ciprofloxacin, levofloxacin, ceftriaxone, and chloramphenicol) against clinical multidrug-resistant strains of P. aeruginosa and E. cloacae by arbutin.
Materials and methods. The molecular docking was performed using AutoDockTools 1.5.6; antimicrobial effects were evaluated by the well method. Isolates were obtained from clinical samples including tracheal aspirate and broncoalveolar lavage.
Results. Theoretical studies have found that none of the investigated antibiotics and arbutin highly selectively inhibit all "targets" mechanisms of antimicrobial action. In experimental studies, it was observed that adding arbutin to the antibiotic led to the emergence of sensitivity on the part of resistant strains. All Gramm-negative resistance strains of bacteria were sensitive to the action of arbutin. Moreover, arbutin increased the antimicrobial effect of antibiotics from 8 to 55 %. It was estimated exceptions such as clarithromycin and azithromycin when assessing antimicrobial activity against P. aeruginosa.
Conclusions. These studies have shown that inhibiting resistant strains of bacteria requires the use of…
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