Determination of antimicrobial activity of fruits of citrullus colocynthis
Abstract
Objective: The current study was designed to examine the antibacterial activity of various extracts of Citrullus colocynthis fruits against Helicobacter pylori, Salmonella enterica, Shigella flexneri, Campylobacter jejuni, Micoplasma pneumonia, and Shigella dysenteriae.Methodology: Antimicrobial activity was evaluated by agar disc diffusion method. The available genome of C. colocynthis was utilized to retrieve sequence of highly conserved genes matK, rpl20, rpl32, and rps7 and to identify their relevant genes in C. rehmii.Results and Conclusion: The maximum antimicrobial activity was shown by ethanol extract (S. f. 32mm, C. j. 27mm, H. p. 26mm, S. d. 22mm, M. p. 21mm, S. e. 19mm) while least activity was given by n-hexane extract (S. f. 13mm, C. j. 11mm, H. p. 18mm, S. d. 8mm, M. p. 10mm, S. e. 8mm) when compared with standard antibiotic levofloxacin (S. f. 30mm, C. j. 30mm, H. p. 30mm, S. d. 28mm, M. p. 25mm, S. e. 20mm). All extracts had given maximum response against H. pylori and S. flexneri and least response against M. pneumoniae and S. enterica. The in-silico genome-wide investigation studies showed that all four genes of C. rehmii were identified with high conservation based on motif distribution and phylogenetic relations.
Read the paper
Related studies
- Severe clinical outcomes of COVID-19 associated with proton pump inhibitors: a nationwide cohort study with propensity score matching
- Ferulic acid regulates the AKT/GSK-3β/CRMP-2 signaling pathway in a middle cerebral artery occlusion animal model
- Association between cord blood 25-hydroxyvitamin D concentrations and respiratory tract infections in the first 6 months of age in a Korean population: a birth cohort study (COCOA)
- Novel Pharmacokinetic/Pharmacodynamic Parameters Quantify the Exposure–Effect Relationship of Levofloxacin against Fluoroquinolone-Resistant Escherichia coli
- Neuroprotective Effects of Epicatechin against Oxidative Stress-Induced Cognitive Impairment: A Systematic Review and Meta-Analysis
- Photochemical behavior of levofloxacin
Go deeper in Evidence Hub
Evidence Hub adds a study data sheet (design, sample size, evidence level, key numbers), an AI summary and a chat grounded in the paper's text.