Design and Development of a Bimodal Optical Instrument for Simultaneous Vibrational Spectroscopy Measurements
Abstract
Vibrational spectroscopy techniques are widely used in analytical chemistry, physics and biology. The most prominent techniques are Raman and Fourier-transform infrared spectroscopy (FTIR). Combining both techniques delivers complementary information of the test sample. We present the design, construction, and calibration of a novel bimodal spectroscopy system featuring both Raman and infrared measurements simultaneously on the same sample without mutual interference. The optomechanical design provides a modular flexible system for solid and liquid samples and different configurations for Raman. As a novel feature, the Raman module can be operated off-axis for optical sectioning. The calibrated system demonstrates high sensitivity, precision, and resolution for simultaneous operation of both techniques and shows excellent calibration curves with coefficients of determination greater than 0.96. We demonstrate the ability to simultaneously measure Raman and infrared spectra of complex biological material using bovine serum albumin. The performance competes with commercial systems; moreover, it presents the additional advantage of simultaneously operating Raman and infrared techniques. To the best of our knowledge, it is the first demonstration of a combined Raman-infrared system that can analyze the same sample volume and obtain optically sectioned Raman signals. Additionally, quantitative comparison of confocality of backscattering micro-Raman and off-axis Raman was performed…
Read the paper
Related studies
- Lathyrism-induced alterations in collagen cross-links influence the mechanical properties of bone material without affecting the mineral
- High-Resolution SPECT-CT/MR Molecular Imaging of Angiogenesis in the Vx2 Model
- Severely impaired bone material quality in Chihuahua zebrafish resembles classical dominant human osteogenesis imperfecta
- Small-animal SPECT and SPECT/CT: application in cardiovascular research
- Intravital imaging of osteocytes in mouse calvaria using third harmonic generation microscopy
- Techniques and Evaluation From a Cross-Platform Imaging Comparison of Quantitative Ultrasound Parameters in an In Vivo Rodent Fibroadenoma Model
Go deeper in Evidence Hub
Evidence Hub adds a study data sheet (design, sample size, evidence level, key numbers), an AI summary and a chat grounded in the paper's text.