Phytochemistry and Biological Activities of Hibiscus sabdariffa Leaf Extract: A Comprehensive Analysis and In Silico Approaches
Abstract
Gas chromatography-mass spectrometry (GC-MS) and liquid chromatography-mass spectrometry (LC-MS) methods were used to simultaneously isolate and determine phytochemicals in the Hibiscus sabdariffa leaf extract. GC-MS analysis revealed the presence of oleic acid (52.05%), hexadecenoic acid (19.78%), and docosenoic acid (7.62%). LC-MS analysis identified six flavonoids and four phenolic acids. Quantitative analysis of some identified phenolic compounds (mg/g in dried leaves) showed the presence of quercetin-rutinoside (11.87), neochlorogenic acid (5.64), and quercetin (4.75). The total phenolic content of the extract, determined using the Folin-Ciocalteu assay, was 22.2 mg GAE/g. The in vitro antioxidant activity was measured by the ABTS radical cation decolorization assay (135.7 µmol trolox/g). The antibacterial activity was evaluated using the disc diffusion method against three Gram-positive bacteria and three Gram-negative bacteria. The leaves extract demonstrated antibacterial activity against all tested bacteria, with inhibition diameters ranging from 8 to 28 mm. The binding energies for ciprofloxacin, levofloxacin, and quercetin, ranging from −5.2 to −5.8, suggest strong interactions with the B subunit of DNA gyrase and the ParE subunit of topoisomerase IV. The study indicates that H. sabdariffa leaf extract has potent antioxidant and antibacterial properties. Additionally, quercetin from H. sabdariffa shows antibacterial potential against DNA gyrase and topoisomerase.
Read the paper
Related studies
- Ferulic acid regulates the AKT/GSK-3β/CRMP-2 signaling pathway in a middle cerebral artery occlusion animal model
- Novel Pharmacokinetic/Pharmacodynamic Parameters Quantify the Exposure–Effect Relationship of Levofloxacin against Fluoroquinolone-Resistant Escherichia coli
- Photochemical behavior of levofloxacin
- Correlation Between Dissolution Profiles of Salt-Form Drugs in Biorelevant Bicarbonate Buffer and Oral Drug Absorption: Importance of Dose/ Fluid Volume Ratio
- Evaluation of the synergistic potential and mechanisms of action for de novo designed cationic antimicrobial peptides
- Synthesis of [11C]levofloxacin
Go deeper in Evidence Hub
Evidence Hub adds a study data sheet (design, sample size, evidence level, key numbers), an AI summary and a chat grounded in the paper's text.