Isolation and Characterization of Heterotrophic Marine Protists for Potential PUFA Production from Nothern coast of East Java and Madura Island
Abstract
Abstract Polyunsaturated Fatty Acids (PUFAs), particularly omega-3s, are essential for human health, yet traditional fish oil sources face critical sustainability challenges. This study explores alternative omega-3 sources from marine oleaginous protists isolated from mangrove ecosystems on the Nothern coast of East Java (Probolinggo/Situbondo) and Madura Island (Bangkalan), Indonesia. Using the pine pollen baiting method, the research aimed to isolate, identify, and compare the lipid-producing capabilities of indigenous strains. While initial morphological screening suggested the presence of Thraustochytrids in all locations, 18S rRNA gene sequencing revealed distinct taxonomic identities. The isolate from the Nothern coast of East Java (ZN2L) was identified as Thraustochytrids, whereas the isolate from Madura (ZN7L) was identified as Crypthecodiniaceae sp. (heterotrophic Dinoflagellates). Despite their evolutionary divergence, both strains exhibited functional convergence with rapid heterotrophic growth and significant lipid accumulation on agro-industrial waste. Quantitative analysis revealed that the Java isolate produced 17.95 mg/g of Docosahexaenoic Acid (DHA), a yield comparable to the 18.25 mg/g achieved by the Madura isolate when cultivated on lemon peel waste substrate. Notably, the Madura isolate also demonstrated the highest specific growth rate (0.127 h − 1). These findings highlight the effectiveness of the baiting method in capturing diverse lineages of the…
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