Omega-3 supplementation increases 13C-EPA but not 13C-DHA kinetics in healthy older adults after 13C-DHA single dose intake: A secondary analysis
Abstract
OBJECTIVE
To investigated whether post supplementation, 13C-DHA is less incorporated into plasma phospholipids (PL) and triglycerides (TG) compared to pre-supplementation considering that these are the two most prominent delivery pools of DHA to tissues.
METHODOLOGY
Plasma samples from twenty healthy men and women, matched for age and body mass index, were analyzed. Participants received a single oral dose of 40 mg 13C-DHA in 2 kinetic studies: 1 prior to and 1 during the last month of supplementation with 3.2 g/day of ω3 PUFA. 13C enrichment analyses were performed on plasma samples at baseline, 4, 6, 24 hours, 7 days, and 28 days post-tracer intake. Lipids were extracted, and PL and TG were separated by solid-phase extraction. Quantification of carbon-13 eicosapentaenoic acid (13C-EPA) and 13C-DHA was performed using gas chromatography-mass spectrometry (GC-MS).
RESULTS
The area under the curve (AUC) for 13C-DHA in PL and TG did not show statistically significant differences before and during supplementation. However, the AUC for 13C-EPA in PL and TG increased by 45% and 48% with supplementation, respectively. This finding was unexpected, as our previous analysis indicated a 60% reduction in plasma 13C-DHA levels during supplementation, accompanied by increased β-oxidation. Additionally, there were statistically significant time × gender interactions for 13C-EPA and 13C-DHA in plasma PL (P < 0.0001), with women exhibiting 90% and 139% higher levels of 13C-EPA and 13C-DHA…
How strong is the evidence?
Evidence Hub reads this as a secondary / pooled analysis, done in people. Its evidence level is 3 of 5 (Moderate · trial / cohort / secondary analysis). Trials without a large randomized design, cohorts and secondary analyses give moderate evidence.
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