A Novel Bioluminescence Tomography System Compatible with CBCT-guided Small Animal Irradiators for High-Precision Preclinical Radiation Research
Abstract
Widely used cone-beam computed tomography (CBCT)-guided irradiators struggle to localize soft-tissue targets due to low imaging contrast. While bioluminescence tomography (BLT) offers a promising functional imaging solution, its adoption in pre-clinical radiotherapy research has been limited. To address this, we developed MuriGlo, a novel BLT system compatible with CBCT-guided small animal irradiators to support high-precision radiation studies. We demonstrate MuriGlo's capabilities in supporting both in vitro and in vivo experiments. MuriGlo consists of a detachable mouse bed, thermostatic control, mirrors, filters, and a charge-coupled device (CCD) camera, enabling multi-projection and multi-spectral bioluminescence imaging (BLI). The detachable bed facilitates animal transfer between MuriGlo and an irradiator for BLT-guided radiation study. We evaluated the thermostatic control's ability and demonstrated that it can maintain a consistent animal body temperature at 37°C throughout imaging. We also quantified detection sensitivity via signal-to-noise ratio (SNR) in detecting minimal cell quantities using glioblastoma (GL261) cells with Luc2 and AkaLuc reporters. The optical system can detect as few as 1173 GL261-Luc2 and 61 GL261-AkaLuc cells in vitro at SNR = 5. For image-guided capabilities, we present BLT-guided 5-arc, BLT-guided 2-field box, and BLI-guided single-field plans. The high conformal 5-arc plan fully covers gross tumor volume (GTV) at prescribed dose with…
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