Folic Acid Targeting Shared Sepsis-Osteoporosis Pathogenesis: From Computational Discovery to Dynamic Binding Mechanisms
Abstract
Sepsis and osteoporosis (OP) are two common diseases that have a significant impact on public health. This study aims to elucidate the common genetic factors and molecular mechanisms underlying the relationship between sepsis and osteoporosis and to explore novel treatment methods. We employed a comprehensive bioinformatics approach, utilizing gene expression data from multiple human databases. This approach included differential expression analysis, weighted gene coexpression network analysis (WGCNA), enrichment analysis, machine learning, immune infiltration analysis, and molecular docking and molecular dynamics simulations. Our study identified four key genes, CTSD, RNASE2, KYNU, and CX3CR1, that are associated with both sepsis and OP. Functional enrichment analysis revealed significant connections to immune processes and signaling pathways, including autophagy and the IL-17 signaling pathway. A nomogram constructed from these four genes demonstrated a strong diagnostic performance for OP, achieving an area under the curve (AUC) of 0.797 (95%CI: 0.712-0.882). Additionally, the validation data set for sepsis demonstrated an even higher AUC of 0.903 (95%CI: 0.825-0.980). Moreover, the immune infiltration analysis revealed significant correlations between these key genes and various immune cell types, suggesting that modulating the immune cell activity could be a potential therapeutic target. Furthermore, molecular docking studies identified folic acid as a promising…
Read the paper
Related studies
- eggNOG 5.0: a hierarchical, functionally and phylogenetically annotated orthology resource based on 5090 organisms and 2502 viruses
- Online Survival Analysis Software to Assess the Prognostic Value of Biomarkers Using Transcriptomic Data in Non-Small-Cell Lung Cancer
- OptiType: precision HLA typing from next-generation sequencing data
- Nicotinic Cholinergic System and COVID-19: In Silico Identification of an Interaction between SARS-CoV-2 and Nicotinic Receptors with Potential Therapeutic Targeting Implications
- Saccharomyces cerevisiae cells have three Omega class glutathione S-transferases acting as 1-Cys thiol transferases
- Hemolysis at low blood flow rates: in-vitro and in-silico evaluation of a centrifugal blood pump
Go deeper in Evidence Hub
Evidence Hub adds a study data sheet (design, sample size, evidence level, key numbers), an AI summary and a chat grounded in the paper's text.