In vitro efficacy of colistin against multidrug-resistant Pseudomonas aeruginosa in burn patient by minimum inhibitory concentration with broth dilution
Abstract
Background: Pseudomonas aeruginosa strains cause 86% of sepsis mortality in burn victims. Therefore, to combat the multi-drug resistance in Pseudomonas aeruginosa, colistin is the new drug, and it has recently been introduced. To analyze the in vitro efficacy of colistin against multidrug-resistant Pseudomonas aeruginosa in burn patients by determining the minimum inhibitory concentration with broth dilution.
Material And Methods: The cross-sectional study was performed from March 2021 to February 2022 in the Burn Centre at Nishter Hospital in Multan, Pakistan. 300 burn patients (≥20% burn) were selected, and their pus samples were collected and processed in the microbiology laboratory. The Kirby-Bauer disc diffusion method was applied to check the antibiotic susceptibility against isolated strains. The colistin sensitivity against multi-drug resistant (MDR) strains was estimated by employing the broth dilution method at two-fold serial dilutions from 0.5 µg/mL to 0.003 µg/mL.
Results: 124 (55.3%) strains were identified as Pseudomonas aeruginosa, while the remaining strains were identified as Escherichia coli (13.0%), Streptococci (13.0%), Klebsiella pneumoniae (8.9%), Staphylococci (6.6%), and Candida albicans (2.2%). All isolated strains of Pseudomonas aeruginosa showed resistance against antibiotics: aztreonam (60.4%), cefepime (72.5%), ceftazidime (71.7%), ciprofloxacin (62.0%), imipenem (33.0%), levofloxacin (58.0%), meropenem (19.3%), piperacillin/tazobactam (66.1%),…
How strong is the evidence?
Evidence Hub reads this as a cross-sectional, done in people. Its evidence level is 4 of 5 (Low · observational). Observational designs can show an association but not prove cause. Reported size: 300 participants or records.
Read the paper
Related studies
- Penicillins for Treatment of Pneumococcal Pneumonia: Does In Vitro Resistance Really Matter?
- Thiophenes—Naturally Occurring Plant Metabolites: Biological Activities and In Silico Evaluation of Their Potential as Cathepsin D Inhibitors
- Inhalable Mannosylated Rifampicin–Curcumin Co-Loaded Nanomicelles with Enhanced In Vitro Antimicrobial Efficacy for an Optimized Pulmonary Tuberculosis Therapy
- Development and validation of microbial bioassay for quantification of Levofloxacin in pharmaceutical preparations
- GC-MS Analysis and Biomedical Therapy of Oil from n-Hexane Fraction of Scutellaria edelbergii Rech. f.: In Vitro, In Vivo, and In Silico Approach
- Propranolol, chlorpromazine and diclofenac restore susceptibility of extensively drug-resistant (XDR)-Acinetobacter baumannii to fluoroquinolones
Go deeper in Evidence Hub
Evidence Hub adds a study data sheet (design, sample size, evidence level, key numbers), an AI summary and a chat grounded in the paper's text.