Fluorescence Imaging of Neuroblastocyte Differentiation and Parkinson's Brain Tissue Lesions Examined by a Norepinephrine-Activated Molecular Probe
Abstract
In living organisms, in situ labeling of norepinephrine (NE) to visualize neuroregulatory mechanisms is of significant importance for the pathological analysis of neurological diseases. Traditional "extraction-detection" methods lack spatiotemporal precision and are disconnected from the dynamic changes of life processes. Fluorescent probes combined with high-resolution microscopy imaging techniques have shown outstanding advantages in biological in situ imaging research. Here, we have developed a NE-specific recognition molecular probe, DQF, by covalently linking the high molar extinction coefficient black-hole quencher BBQ-650 with a fluorescent reporter group, utilizing the fluorescence resonance energy transfer mechanism for prequenching fluorescence. The probe DQF features low self-fluorescence (enhancing the contrast before and after fluorescence activation), long-wavelength emission (658 nm, improving the signal-to-noise ratio), and a sensitive, specific response to NE (16 min, 58.6 μM). Compared to the previously developed probe SXU-NEQ, the self-fluorescence intensity of probe DQF decreased significantly (IDQF/ISXU-NEQ = 35%). Time-dependent density functional theory confirmed the differences in fluorescence quenching mechanisms between the two probes. Confocal cell imaging and flow cytometry experiments confirmed that DQF can sensitively label changes in NE levels in SH-SY5Y neuroblast cells during differentiation. Additionally, probe DQF was applied for imaging NE…
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