Syrian Golden Hamsters — a Model for Assessing the Efficacy of Antiviral Drugs Against Experimental COVID-9
Abstract
Introduction. The viral disease COVID-19 has caused a global emergency and attracted the attention of health professionals and the public worldwide. The significant increase in the number of new cases of infection with this virus demonstrates the relevance of finding medications effective against this pathogen. A laboratory model is needed to assess the activity of antiviral drugs. The aim of this study was to develop an experimental model that adequately reproduces the leading syndrome of the human disease COVID-19. Material and methods. Research methods: experimental and analytical. The experiments were conducted using a permanent culture of African green monkey kidney cells — Vero Cl008, as well as Syrian golden hamsters orally infected with various variants of the SARS-CoV-2 virus. Results. A model of the experimental form of COVID-19 in Syrian golden hamsters with oral infection was developed based on a set of parameters (clinical, virological, hematological, and biochemical). It was shown that as a result of oral infection at a dose of 500 ID50 animals developed lung damage, a decrease in the number of leukocytes in the blood, an increase in the proportion of young and band forms of neutrophils with a simultaneous decrease in the proportion of segmented neutrophils, lymphocytes, an increase in the activity of aspartate and alanine aminotransferases (AST and ALT), an increase in the concentration of urea, creatinine, lactate dehydrogenase (LDH), and creatine…
Read the paper
Related studies
- Penicillins for Treatment of Pneumococcal Pneumonia: Does In Vitro Resistance Really Matter?
- Thiophenes—Naturally Occurring Plant Metabolites: Biological Activities and In Silico Evaluation of Their Potential as Cathepsin D Inhibitors
- Inhalable Mannosylated Rifampicin–Curcumin Co-Loaded Nanomicelles with Enhanced In Vitro Antimicrobial Efficacy for an Optimized Pulmonary Tuberculosis Therapy
- Development and validation of microbial bioassay for quantification of Levofloxacin in pharmaceutical preparations
- GC-MS Analysis and Biomedical Therapy of Oil from n-Hexane Fraction of Scutellaria edelbergii Rech. f.: In Vitro, In Vivo, and In Silico Approach
- Propranolol, chlorpromazine and diclofenac restore susceptibility of extensively drug-resistant (XDR)-Acinetobacter baumannii to fluoroquinolones
Go deeper in Evidence Hub
Evidence Hub adds a study data sheet (design, sample size, evidence level, key numbers), an AI summary and a chat grounded in the paper's text.