In vitro evaluation of stability and hemostatic efficacy of single-donor lyophilized canine plasma
Abstract
Introduction Interest in lyophilized plasma products has increased. However, data on their use in dogs are limited. This study aimed to evaluate the in vitro stability and hemostatic efficacy of single-donor lyophilized canine plasma. Methods Ten canine plasma units were lyophilized and stored at −80 °C, 4 °C, room temperature, and 38 °C for 45 days. The plasma compositions before and after lyophilization were compared to assess the impact of the lyophilization. The following parameters were assessed to evaluate storage stability: blood gas analysis, biochemical parameters, coagulation profiles [prothrombin time (PT); activated partial thromboplastin time (aPTT); fibrinogen concentration; the activities of coagulation factors II, V, VIII, IX, X, and XII, as well as those of antithrombin (AT); and protein C], and kaolin-activated thromboelastography. Aerobic bacterial cultures were performed using thioglycollate broth to assess sterility. Lyophilized plasma samples were reconstituted to 50, 60, 80, and 100% of the original plasma volume to assess the effects of different reconstitution volumes on plasma components. Total protein, albumin, osmolality, selected coagulation factors (II and V), fibrinogen, and AT were measured and compared across the reconstituted groups. Results Lyophilization decreased the partial pressure of carbon dioxide and increased the pH. No other significant immediate changes were observed. Plasma stored at −80 °C and 4 °C maintained stable biochemical…
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