Protective effect of magnesium isoglycyrrhizinate combinated with verapamil on liver ischemia-reperfusion injury after semi-hepatectomy in rats
Abstract
OBJECTIVE: To investigate protective effect of isoglycyrrhizinate combinated with verapamil on liver ischemia-reperfusion injury after semi-hepatectomy in rats.
METHODS: Male SD rats were randomly divided into five groups: false operation (group A); rats subjected to 50％hepatectomy and 45 min of hepatic ischemia (group B); rats subjected to 50% hepatectomy and 45 min of hepatic ischemia, and treated with isoglycyrrhizinate (group C); rats subjected to 50% hepatectomy and 45 min of hepatic ischemia, and treated with verapamil (group D); rats subjected to 50% hepatectomy and 45 min of hepatic ischemia, and treated with isoglycyrrhizinate and verapamil (group E). Liver function, as well as morphology, SOD activity, FasL, caspase 3, and β-catenin expression of rats were analyzed.
RESULTS: Compared to group B, the levels of serum ALT of group E were significantly lower (P<0.05), SOD activity of liver tissue (98.8±7.1) were significantly higher (P<0.01), pathological changes were milder, FasL gene (1.327±0.193) and protein (0.010 9±0.001 4) expression were significantly lower (P<0.01). Caspase 3 protein expression levels (0.141 0±0.005 3) were significantly lower (P<0.001), β-catenin protein expression levels (0.079 1±0.008 2) were higher significantly (P<0.01).
CONCLUSIONS: Isoglycyrrhizinate combinated with verapamil can effectively reduce rats liver ischemia-reperfusion injury. The protective effect on liver cells may be mediated by inhibiting apoptosis caused by Fas/FasL…
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