Antioxidant, Anti-inflammatory, and Anti-wrinkle Effects of Sargassum horneri Extract in vitro
Abstract
Purpose: This study mainly aimed to confirm the antioxidant activity, antiinflammatory, and collagen production enhancement effects of the Sargassum horneri (SH) extract.Methods: The measurement of the total flavonoid and total polyphenol content, 2,2′-azino-bis-3-ethylbenzthiazoline-6-sulphonic acid (ABTS), and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activities confirmed the antioxidant efficacy of the SH extract. The expression level of interleukin 6 (IL-6) and its upstream mechanism pathway, mitogen-activated protein kinases (MAPK), were evaluated for anti-inflammatory efficacy in human dermal fibroblast (HDF) cells. The collagenase and elastase levels were measured in terms of the ability to inhibit collagen degradation. On the contrary, collagen type 1 (COL1) and matrix metallopeptidase 1 (MMP1) were conducted to confirm the collagen production ability.Results: The DPPH and ABTS radical scavenging abilities of the SH extract were 86.87%±12% and 76.49%±2.94%, respectively, at the highest concentration of 1000 μg/mL, confirming 51.62±1.945 and 79.65±4.732 mg/g of total polyphenol and total flavonoid, respectively, which are known to have antioxidant effects. The anti-inflammatory effect of SH in HDF cells was reduced by up to 56.8% compared to the control group due to a significant decrease in MAPK. The SH extract significantly reduced collagenase and elastase, thereby inhibiting collagen degradation. On the contrary, increasing COL1 and decreasing MMP1 in…
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