Isolation and characterization of a novel lytic phage Artin targeting typhoidal and nontyphoidal Salmonella and evaluation of its antibacterial efficacy in food
Abstract
Background: Phages are increasingly viewed as powerful natural tools for controlling Salmonella and other foodborne pathogens, offering an alternative to conventional antimicrobials. However, the extensive serotype diversity of Salmonella and the rise of phage-resistant strains highlight the need for isolating phages with broader host ranges and strictly lytic activity. Advancing such phage resources is essential for developing reliable biocontrol strategies.
Methods: In this study, a novel lytic phage with significant potential to combat various Salmonella strains was isolated from dog feces. Morphological and biological characteristics of phage Artin were assessed using transmission electron microscopy, stability assays under different temperature and pH conditions, and adsorption assays. Structural proteins were analyzed by SDS-PAGE. The host range was determined against four Salmonella serovars and six non-Salmonella species. Whole-genome sequencing followed by bioinformatics analysis was performed to elucidate the genomic features of Artin. The ability of phage Artin to control Salmonella Enteritidis in chicken breast meat and milk was further assessed at 4 °C. Statistical analysis was carried out using GraphPad Prism 8.0.
Results: Genomic analysis classified Salmonella_phage_Artin within the Caudoviricetes class, Markadamsvirinae subfamily, and Epseptimavirus genus, with a dsDNA genome size of 118,477 bp and a GC content of 39.78%, featuring 192 coding sequences (CDS)…
Read the paper
Related studies
- Ferulic acid regulates the AKT/GSK-3β/CRMP-2 signaling pathway in a middle cerebral artery occlusion animal model
- Novel Pharmacokinetic/Pharmacodynamic Parameters Quantify the Exposure–Effect Relationship of Levofloxacin against Fluoroquinolone-Resistant Escherichia coli
- Photochemical behavior of levofloxacin
- Correlation Between Dissolution Profiles of Salt-Form Drugs in Biorelevant Bicarbonate Buffer and Oral Drug Absorption: Importance of Dose/ Fluid Volume Ratio
- Evaluation of the synergistic potential and mechanisms of action for de novo designed cationic antimicrobial peptides
- Synthesis of [11C]levofloxacin
Go deeper in Evidence Hub
Evidence Hub adds a study data sheet (design, sample size, evidence level, key numbers), an AI summary and a chat grounded in the paper's text.