Antibiofilm activity of chlorhexidine and levofloxacin on pathogen causing orthopaedics implant-related infections
Abstract
Objectives: The incidence of hospital-acquired infections (HAIs) related to the formation of biofilms reaches 80% of the total cases of infection in the world. It can infect patients using invasive medical equipment such as orthopaedics implants. Biofilm-related implant orthopaedics infections account for approximately 65% of all bacterial infections. Once bacteria adhere to the implant, a bacterial community established biofilm that can enhance resistance to antibiotics up to 1000 times. Therefore, an appropriate strategy is needed to eradicate biofilm. Chlorhexidine as an antiseptic and levofloxacin as an antibiotic are often used in the orthopaedics’ setting. This study investigated in vitro antibiofilm activity of chlorhexidine and levofloxacin against bacterial isolates obtained from patient with implant orthopaedics-related infections. Methods: Ten clinical isolates of bacteria with strong biofilm-producer were collected from patients with orthopaedics implant-related infections including Staphylococcus aureus (n=2), Staphylococcus haemolyticus (n=1), Serratia marcescens (n=2), Pseudomonas aeruginosa (n=2), Proteus mirabilis (n=1), Acinetobacter baumannii (n=1) and Klebsiella pneumoniae (n=1). The inhibition and eradication activity of chlorhexidine and levofloxacin on biofilm growth were performed using microtiter broth dilution method in 96-well plates. The minimum biofilm inhibitory concentration (MBIC) and minimum biofilm eradication concentration (MBEC) were…
Read the paper
Related studies
- Ferulic acid regulates the AKT/GSK-3β/CRMP-2 signaling pathway in a middle cerebral artery occlusion animal model
- Novel Pharmacokinetic/Pharmacodynamic Parameters Quantify the Exposure–Effect Relationship of Levofloxacin against Fluoroquinolone-Resistant Escherichia coli
- Photochemical behavior of levofloxacin
- Correlation Between Dissolution Profiles of Salt-Form Drugs in Biorelevant Bicarbonate Buffer and Oral Drug Absorption: Importance of Dose/ Fluid Volume Ratio
- Evaluation of the synergistic potential and mechanisms of action for de novo designed cationic antimicrobial peptides
- Synthesis of [11C]levofloxacin
Go deeper in Evidence Hub
Evidence Hub adds a study data sheet (design, sample size, evidence level, key numbers), an AI summary and a chat grounded in the paper's text.