Molecular epidemiology of Escherichia coli in bloodstream infections from a general hospital in Ningxia, China, 2022-2023
Abstract
OBJECTIVE: To analyse the antibiotic resistance, resistance genes and clonal relationship of Escherichia coli in bloodstream infections in Ningxia from 2022 to 2023.
METHODS: We retrospectively analyzed the antibiotic susceptibilities of 257 isolates. PCR was used to detect blaTEM, blaSHV, blaCTX-M, qnrS, qnrA, qnrB, oqxA, qepA, gyrA, gyrB, parC, and parE, and the clonal relationship through multilocus sequence typing (MLST).
RESULTS: One hundred and twenty-nine of 257 patients were male (50.2%). The 257 E. coli isolates were mainly obtained from the Emergency, Hepatobiliary Surgery, and Haematology Departments, accounting for 56.6%, 7.3%, and 6.2%, respectively. There is no significant difference in sex and genes between the two groups over and under 60 years old (P > 0.05), but there is a significant difference in ST between them(P<0.05). The antimicrobial susceptibility testing showed that the 257 isolates had the highest rates of resistance to ampicillin (82.8%), followed by cefazolin (71.6%), and all isolates were susceptible to tigecycline. Based on the antibiotic susceptibility results for ceftriaxone, we tested 126 isolates of E. coli for extended-spectrum beta-lactamase (ESBL) resistance genes. As a result, blaCTX-M was detected in 76 isolates (60.32%), blaSHV in 26 isolates (20.63%), and blaTEM in 38 isolates (30.16%). Based on the ciprofloxacin and levofloxacin antibiotic susceptibility results, we tested for quinolone resistance genes in 148 isolates, revealing…
How strong is the evidence?
Evidence Hub reads this as a cohort study, done in people. Its evidence level is 3 of 5 (Moderate · trial / cohort / secondary analysis). Trials without a large randomized design, cohorts and secondary analyses give moderate evidence. Reported size: 257 participants or records.
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