Investigation of metronidazole resistance-associated mutations and virulence genotypes in helicobacter pylori isolates from the Egyptian population: A cross-sectional study
Abstract
INTRODUCTION: This cross-sectional study assesses the prevalence of metronidazole resistance-associated mutations and virulence genotypes in Helicobacter pylori (H. pylori) strains isolated from the Egyptian population. H. pylori infection is a significant public health concern, with antibiotic resistance challenging its eradication.
METHODS: Gastric biopsy samples were collected from symptomatic patients referred for upper gastrointestinal endoscopy at selected healthcare facilities. The study included 250 participants with symptoms suggestive of H. pylori infection and aged 18 years or older. Biopsy samples were obtained using standard endoscopic techniques, and H. pylori strains were isolated and identified in the laboratory. Antimicrobial susceptibility testing was conducted using standard methods. Molecular analysis, including polymerase chain reaction (PCR) and sequencing, was performed to identify metronidazole resistance-associated mutations (rdxA and frxA) and virulence genotypes (cagA and vacA).
RESULTS: Antimicrobial susceptibility testing revealed that 43.6 % of the isolates were resistant to metronidazole, while 11.8 %, 4.5 %, and 55.4 % were resistant to clarithromycin, amoxicillin, and levofloxacin. Molecular analysis identified rdxA and frxA mutations in 36.3 % and 31.8 % of the isolates, respectively, indicating metronidazole resistance-associated mutations. Additionally, 60.0 % of the isolates were positive for the cagA gene, and 80.0 % had the vacA s1…
How strong is the evidence?
Evidence Hub reads this as a cross-sectional, done in people. Its evidence level is 4 of 5 (Low · observational). Observational designs can show an association but not prove cause. Reported size: 250 participants or records.
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