Serotonin Receptor Signaling Promotes Amoeboid Migration of Melanoma Cells Through cAMP/PKA Activation
Abstract
Melanoma cells frequently utilize bleb-driven amoeboid migration to navigate confined microenvironments during metastasis. Through a morphology-based drug repurposing screen, we identified the anti-migraine medication dihydroergotamine (DHE) as a potent inhibitor of this behavior. We show that DHE suppresses amoeboid motility by targeting serotonin receptors, primarily 5-HTR7. Trace levels of peripheral serotonin activates 5-HTR7, which in turn stimulates cAMP/PKA and ROCK signaling. This signaling cascade increases the cortical contractility necessary to sustain blebbing. Accordingly, DHE treatment or PKA inhibition significantly impairs confined migration without altering cell proliferation. Clinically, elevated levels of serotonin-degrading enzymes, such as MAOB, correlate with favorable patient prognoses and are reduced in metastatic lesions. Ultimately, these findings reveal an unanticipated role for peripheral serotonin in melanoma biomechanics and highlight serotonin receptors as highly actionable targets to limit metastatic dissemination.
Significance Statement: Metastasis is the leading cause of melanoma-related mortality, driven in part by the ability of tumor cells to adopt a highly contractile amoeboid mode of migration that enables movement through spatially confined tissues. This study uncovers a previously unrecognized mechanobiological role for peripheral serotonin in promoting this invasive behavior. We show that low concentrations of serotonin activate…
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