Diagnosis of intestinal tuberculosis in patient with ileocolonic ulceration using gene Xpert-MTB/RIF assay at A tertiary care hospital IGIMS Patna, Bihar
Abstract
BACKGROUND
Tuberculosis (TB) persists as a significant challenge in global scenario, with developing country like India bearing the relatively more burden. "Intestinal TB (ITB), a form of Extrapulmonary TB (ETB)," is responsible for 12.8 % of extrapulmonary cases and poses diagnostic difficulties due to nonspecific symptoms and similarities to other gastrointestinal diseases like Crohn's disease. Conventional methods, like histopathology and culture, have constrained sensitivity and turnaround time. "The GeneXpert-MTB/RIF assay," a molecular test with rapid results, shows promise in TB diagnosis, but its efficacy for ITB remains understudied. This study sought to evaluate how well the GeneXpert-MTB/RIF assay performs diagnostically in patients with ileocolonic ulcerations suspected of ITB, comparing its sensitivity, specificity, and predictive values with "conventional techniques like MGIT culture and Line Probe Assay (LPA)."
METHODS
A hospital-based cross-sectional investigation spanning 18 months was undertaken at a tertiary care setting center, involving "100 patients aged ≥18 years with ileocolonic ulcerations on colonoscopy and clinical suspicion of ITB". Intestinal biopsies were analyzed using GeneXpert-MTB/RIF, MGIT culture, and LPA. Statistical analyses included sensitivity, specificity, predictive values, and demographic/clinical correlations.
RESULTS
The GeneXpert-MTB/RIF assay demonstrated 100 % sensitivity and specificity compared to MGIT culture, with perfect…
How strong is the evidence?
Evidence Hub reads this as a cross-sectional, done in people. Its evidence level is 4 of 5 (Low · observational). Observational designs can show an association but not prove cause. Reported size: 100 participants or records.
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