Effects of botulinum toxin type A in a migraine-specific animal model
Abstract
Background: OnabotulinumtoxinA (BoNT/A) is an established treatment for chronic migraine, although the detailed molecular mechanisms underlying its efficacy remain unclear. In this study, we examined the anti-hyperalgesic effects of BoNT/A using an animal model of migraine induced by nitroglycerin (NTG) administration associated with the orofacial formalin test, aiming to enhance our understanding of the modulatory effect of the drug on migraine pain pathways.
Methods: Male rats weighing 235-240 g (n=7 per group) were used. BoNT/A (10 U/kg) was administered unilaterally as a 25 μL bolus into the right upper lip. Rats in the control group received an injection of 25 μL of 0.9% saline. Seven days after BoNT/A injection, rats were administered NTG (10 mg/kg, i.p.) or its vehicle and were subjected to the orofacial formalin test 4 hours later. At the end of the behavioral test, the medulla-pons area and the trigeminal ganglia were collected and processed for RT-PCR analysis.
Results: At the orofacial formalin test, the NTG-treated rats had a more marked nocifensive behavior compared to vehicle-treated animals. BoNT/A pretreatment significantly reduced this behavior. In addition, calcitonin gene-related peptide (CGRP), pituitary adenylate cyclase-activating peptide (PACAP), and vasoactive intestinal peptide (VIP) mRNA levels were higher in the NTG-treated group in trigeminal ganglia on both sides compared to the control group, with CGRP and PACAP mRNA levels being higher on the…
Read the paper
Related studies
- Calcium Ions in the Physiology and Pathology of the Central Nervous System
- AI-powered in silico twins: redefining precision medicine through simulation, personalization, and predictive healthcare
- In silico functional, structural and pathogenicity analysis of missense single nucleotide polymorphisms in human MCM6 gene
- In Silico drug repurposing pipeline using deep learning and structure based approaches in epilepsy
- Targeting serotonin receptors with phytochemicals – an in-silico study
- In silico exploration of anti-prostate cancer compounds from differential expressed genes
Go deeper in Evidence Hub
Evidence Hub adds a study data sheet (design, sample size, evidence level, key numbers), an AI summary and a chat grounded in the paper's text.