Effect of activated autophagy on an animal model of vestibular migraine-like attacks
Abstract
BACKGROUND
Patients with vestibular migraine (VM) experience both dizziness and headache. Our team's previous pilot study has revealed a significant increase in the concentration of the autophagic marker P62 in the blood of VM patients. This finding indicates a close association between cellular autophagic function and VM pathogenesis.
OBJECTIVE
In our study, our objective was to integrate the elements of dizziness and headache, thereby constructing a rat model of vestibular migraine-like attacks (VMa) that could effectively mimic the symptoms of VM. This article will conduct an in-depth exploration into the establishment of the VMa rat model and comprehensively analyze the role of autophagy within both the VMa rat model and VM patients.
METHODS
We established the VMa rat model via intraperitoneal nitroglycerin injection and 2-h variable speed rotation. Assessed VMa rats' pain sensitivity by periorbital mechanical and tail thermal pain thresholds. Evaluated their vestibular function with MS index and balance beam experiments. Measured CGRP, c-fos, P62 and LC3-II/I proteins in TCC and VN by western blot. In clinical experiments, selected same-age patients without comorbidities based on CM and VM criteria. Determined P62 and LC3-II concentrations in peripheral plasma by ELISA. Explored the therapeutic significance of blocking PI3K/AKT/mTOR pathway and autophagy agonist intervention in VM disease.
RESULTS
First, compared VMa model rats with normal SHAM group: periorbital…
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