Effects of Tianshu capsule on plasma NO,NOS,CGRP contents and hemodynamics of migraine animal models
Abstract
Objective To investigate the effects of Tianshu capsule on vasoactive substances in blood plasma or brain and hemodynamics in migraine animal models. Methods After subcutaneously administration of nitroglycerin (NTG) and tube feeding Tianshu capsule, the radioimmunoassay or spectrophotometry was used to measure the plasma contents of calcitonin gene related peptide (CGRP), nitric oxide (NO) and nitric oxide synthase (NOS) in rats.The distribution of NOS1, CGRP in rat nucleus tratus spinalis nervi trigemini was detected by immunohistostaining, and the changes of internal carotid artery flow velocity in rabbit were measured by transcranial Doppler.Results The levels of NO, NOS and CGRP were elevated in the plasma of migraine rats. Tianshu capsule could inhibit these increases, and the moderate-and high-dose had much significant effects (P0.05~0.01). Tianshu capsule could also prevented the decrease of peak systolic velocity of internal carotid artery in rabbit model (P0.05). It was observed by immunohistostaining that increased expression of NOS1 and CGRP in the spinal trigeminal nucleus of migraine rats were depressed by Tianshu capsule (all P0.05).Conclusion Tianshu capsule can modulate the unbalance of vasoactive substances and neurotransmitters during the migraine attacks and it may result in relief of the headache.
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