Activation pattern and modulation of pain related structures in animal models of migraine
Abstract
Summary Migraine is a common neurological disorder with a high social burden and unknown pathomechanism. The trigeminal systems plays a crucial part in the pathological processes of the disease, therefore the knowledge about the functioning and modulation options of this system is essential for developing new therapeutic options against this condition. One of the animal models used in migraine research is the electrical stimulation of the trigeminal ganglion (ESTG). In this model we examined the activation pattern of the brain stem nuclei, previously regarded as migraine generators in human studies. To find new targets modulating the trigeminal nociceptive pathway we examined the effects of Brilliant Blue G-250 (BBG) and probenecid (PROB) on the number of c-Fos immunoreactive cells and on the changes in calcitonin gene-related peptide and neuronal nitric oxide synthase immunoreactivity in the caudal trigeminal nucleus. BBG is a P2X7 receptor antagonist, which receptors were previously shown to be involved in nociceptive processing. PROB is an inhibitor of numerous organic anion transporters and multidrug resistance-associated proteins, and was proposed to be antinociceptive. BBG was tested in the ESTG model in two stimulation paradigms, a mild and a robust one, and in the orofacial formalin test, while PROB was tested in the formalin test only. After ESTG the activation pattern of the brainstem nuclei was not similar to the pattern seen during migraine attacks in human…
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Evidence Hub reads this as a thesis, done in animal, not in people. Its evidence level is 5 of 5 (Very low · case / narrative / animal / in vitro / in silico). Case reports, narrative reviews, and animal, cell or computer-model studies give the weakest evidence for human health.
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