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Antimicrobial Activities of Bio-Oil and Bio-Tar Obtained from Pyrolysis of Cocoa Pod Husk (Theobroma Cacao L.) on Some Selected Pathogenic Organisms

Adesemuyi MF, Adeyemo AJ, Akinola AO, Ogundele JO, Olasupo AD, Adeyemi DO, Lajide L

2024

Abstract

Abstract The use of synthetics antibacterial and antifungal activities of bio tar and biological oil obtained via cocoa pod husk were investigated on seven selected bacteria; Erwinia carotovora, Corynebacterium sopedonium, Staphylococcus aureus, Pseudomonas solanacearium, Salmonella typii, Xanthomonasphaseoli, Clavbactermichinganensi and five selected fungal; Phytophthora megacarrier, Fusarium vasinfectum, Collectotrichum lindinuthianum, Glomerella gossypii, Cercospora sojina isolates, employing the procedures for food poisoning and agar culture dispersion, accordingly, Findings from antimicrobial activity indicated that the bio-oil as well as the bio-tar performed reasonably well against all the isolates with a zone of inhibition ranged from19.17 ± 1.04g to 35.77 ± 0.25c mm. The results of antifungal activities showed that the percentage of mycelia growth inhibition ranged from 80.35 to 99.33% against the isolates compared to the standard Mancozeb (control). The biological oil and biotar from cocoa pods husks were pyrolyzed at 400°C and their antifungal activity was noticeably higher than their antibacterial activity.

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