Aromatized nicotine-containing smoking mixtures as a factor in changing the expression of BRCA1 and kras in tissues of experimental animal
Abstract
To simulate the experiment, a mechanical smoking simulation model was created for aspirating and expelling aerosolized smoking mixture from an e-cigarette (vape) in a 96-liter glass cube. The experiment was conducted on 50 female Wistar rats. The experimental animals were exposed to the smoking mixture (6% nicotine content, 50/50 VG/PG, flavored) by aspirating and expelling the mixture from the e-cigarette (vape) five times, totaling 3.5 liters of aerosol. The animals were exposed to the mixture for 5 minutes.
Following exposure to the smoking mixture, samples (liver, lungs, brain, and blood plasma) were collected in accordance with the experimental design protocol. To achieve this goal, we determined the expression of the Brca1 and Kras proto-oncogenes in comparison with the reference genes β-actin (Actb) and Gapdh using PCR analysis in tissues from intact and experimental rats.
Our study demonstrated that smoking mixtures can affect the genetic machinery of cells by altering the strength of gene expression. These processes can lead to the blocking of tumor suppressor gene expression, which will serve as the basis for proto-oncogene activation (genomic instability) and subsequent tumor growth.
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