Review on Method Development and Validation of Metformin, Empagliflozin and Glimepiride Using UPLC
Abstract
UPLC, also known as ultra performance liquid chromatography, was developed by Waters in 2004 and represents an advanced form of HPLC(1). This method has revolutionized liquid chromatography by catering to particles smaller than 2 μm, leading to enhanced sensitivity, speed, and resolution. Many experts believe that UPLC will eventually supplant traditional HPLC methods(2). The Van Deemter equation underpins this transformative process by establishing a connection between linear velocity and plate height.(1) Operating with UPLC on smaller particles necessitates a higher pressure limit compared to standard HPLC, which typically operates at 6000 psi. In comparison to HPLC, this method reduces mobile phase volume usage by at least 80% and shortens the runtime to approximately 1.5 minutes(3,4). UPLC, a modified version of HPLC, takes advantage of advancements in particle chemistry performance, system architecture, detector design, data processing, and control, resulting in significant enhancements in resolution, sensitivity, and efficiency(3). This also leads to quicker results and reduced solvent usage, making the technology more cost-effective and eco- friendly(4)
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