Investigation of carbapenemase-encoding genes in Burkholderia cepacia and Aeromonas sobria isolates from nosocomial infections in Iraqi patients
Abstract
Burkholderia cepacia and Aeromonas sobria are difficult to eradicate due to their innate resistance to a variety of medications, and cause various diseases. The aim of this study was to investigate the occurrence of carbapenemase genes and patterns of antibiotic resistance in isolates of B. cepacia and A. sobria. Randomly, 120 clinical specimens have been collected in patients with nosocomial infections. Selective media were used to culture ear swabs, urine, burns, wounds and cerebrospinal fluids. According to biochemical tests and the VITEK-2 system, 75 of these demonstrated positive growth with B. cepacia and A. sobria. Metallo-β-lactamase (MBL) synthesis was phenotypically screened using the meropenem-EDTA disc test. The recA gene in B. cepacia and the genes encoding carbapenemase in both species were found using PCR tests. Among the 75 isolates assessed 20 (26.6%) were A. sobria and 55 (73.3%) were B. cepacia. Piperacillin, cefepime, and ceftriaxone showed antimicrobial resistance of 100%, followed by ceftazidime (97.3%), cefazolin (96%), and piperacillin/ tazobactam (94.6%). Intermediate resistance was reported with aztreonam (61.3%), meropenem (49.3%), trimethoprim-sulfamethoxazole (49.3%), gentamicin (46.6%), levofloxacin (44%), and ciprofloxacin (44%). It is important to note minocycline (40%), amikacin (40%) imipenem (36%) and tigecycline (34.6%), had the lowest resistance rates, hence their relatively higher efficacy against the tested isolates. In this…
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