Bromhexine hydrochloride enhances the therapeutic efficacy of tiamulin against experimental Staphylococcus aureus infection in dogs: targeting bacterial virulence, boosting antioxidant defense, and improving histopathology
Abstract
Introduction Staphylococcus aureus is a prominent pathogen capable of causing systemic infections and multi-organ damage, primarily driven by its high virulence and induction of oxidative stress. This study evaluated the therapeutic efficacy of tiamulin alone and in combination with bromhexine in a canine model of systemic S. aureus infection, focusing on oxidative stress biomarkers, bacterial burden, tissue histopathology, and the expression of cardiac and bacterial virulence-related genes. Methods Experimental infection was induced in dogs, except for a healthy control group. Animals were assigned to five groups: uninfected control, infected untreated, tiamulin-treated, bromhexine-treated, and tiamulin plus bromhexine-treated. Oxidative stress was assessed through measurements of malondialdehyde (MDA) and total antioxidant capacity (TAC) in cardiac, hepatic, and renal tissues. Bacterial load was quantified, and minimum inhibitory concentrations (MICs) of the treatments were determined. Quantitative PCR was performed to evaluate the expression of S. aureus virulence genes including hla (alpha-hemolysin), ebpS (extracellular matrix-binding protein S), and icaA (intercellular adhesion A). Histopathological analyses of heart, liver, and kidney tissues were conducted, and hematological and biochemical parameters (total protein, albumin, and globulin) were measured. Cardiac injury markers, cytochrome P450 1B1 ( CYP1B1 ) and interleukin-1 beta ( IL-1β ), were also assessed.…
Read the paper
Related studies
- Penicillins for Treatment of Pneumococcal Pneumonia: Does In Vitro Resistance Really Matter?
- Thiophenes—Naturally Occurring Plant Metabolites: Biological Activities and In Silico Evaluation of Their Potential as Cathepsin D Inhibitors
- Inhalable Mannosylated Rifampicin–Curcumin Co-Loaded Nanomicelles with Enhanced In Vitro Antimicrobial Efficacy for an Optimized Pulmonary Tuberculosis Therapy
- Development and validation of microbial bioassay for quantification of Levofloxacin in pharmaceutical preparations
- GC-MS Analysis and Biomedical Therapy of Oil from n-Hexane Fraction of Scutellaria edelbergii Rech. f.: In Vitro, In Vivo, and In Silico Approach
- Propranolol, chlorpromazine and diclofenac restore susceptibility of extensively drug-resistant (XDR)-Acinetobacter baumannii to fluoroquinolones
Go deeper in Evidence Hub
Evidence Hub adds a study data sheet (design, sample size, evidence level, key numbers), an AI summary and a chat grounded in the paper's text.