Acridocarpus smeathmannii root extracts inhibit human prostate and bladder smooth muscle contraction, porcine arterial vasoconstriction, and cytotoxicity of prostate stromal cells
Abstract
Introduction The limited tolerability and efficacy of synthetic drugs have hindered the effective management of lower urinary tract symptoms (LUTSs). In African traditional medicine, species of the genus Acridocarpus (Malpighiaceae) are commonly used to treat reproductive disorders. In this study, we investigated the bioactivity-guided effects of Acridocarpus smeathmannii on smooth muscle contractility using human tissues obtained from radical prostatectomy and cystectomy procedures, as well as porcine coronary and interlobar arteries. Additionally, the impact of A. smeathmannii on the proliferation of cultured prostate stromal cells was evaluated. Methods Cumulative concentration–response curves were generated for both adrenergic and cholinergic agonists, and electrical field stimulation (EFS) in organ bath experiments. In addition, assays were conducted to evaluate cell proliferation and viability, providing complementary insights into functional and cellular-level effects. The bioactive compounds in the extract were characterized using gas chromatography–mass spectrometry (GC-MS) and nuclear magnetic resonance spectroscopy and were subsequently evaluated in silico for their interaction with the α1-adrenergic receptor. Results Prostate tissue contractions induced by α1-adrenergic agonists (0.1–100 µM) were reduced by 50% or more with A. smeathmannii at concentrations of 0.25 and 0.50 mg/mL. Bladder tissue contractions induced by the cholinergic agonists (0.1–1000 µM) were…
Read the paper
Related studies
- Treatment of non-metastatic castration-resistant prostate cancer: facing age-related comorbidities and drug–drug interactions
- The Efficacy of Flogofilm® in the Treatment of Chronic Bacterial Prostatitis as an Adjuvant to Antibiotic Therapy: A Randomized Prospective Trial
- Inhibition of growth and contraction in human prostate stromal cells by silencing of NUAK1 and -2, and by the presumed NUAK inhibitors HTH01-015 and WZ4003
- Effectiveness and Safety of a New Nutrient Fixed Combination Containing Pollen Extract Plus Teupolioside, in the Management of LUTS in Patients with Benign Prostatic Hypertrophy: A Pilot Study
- Extract from Cucurbita pepo improves BPH symptoms without affecting sexual function: a 24-month noninterventional study
- Aquablation vs. holmium laser enucleation of the prostate for benign prostatic hyperplasia: a 150-patients prospective comparative multicenter study
Go deeper in Evidence Hub
Evidence Hub adds a study data sheet (design, sample size, evidence level, key numbers), an AI summary and a chat grounded in the paper's text.