Ozone-modified properties of pumpkin seed oil as anti-H. pylori, anticancer, anti-diabetic and anti-obesity agent
Abstract
Pumpkin is a widely recognized plant in the Cucurbitaceae family because of its significance as a pharmaceutical food. Ozonized oils are becoming more popular among researchers and being employed in medical facilities due the continuous search for powerful biomedical medicines that can combat illnesses with controllable negative repercussions. Pumpkin seed oil was ozonized at an average rate of 0 to 8 L/minute. The chemical composition of the oil before and after treatment was characterized using Gas Chromatography-Mass Spectrometry (GC-MS) analysis. Anti- Helicobacter pylori (H. pylori) of both unrefined and ozonized pumpkin oils were detected using agar well diffusion technique. The anti-biofilm was tested at 25, 50 and 75% of MBC. Time killing assay was done to compare the antimicrobial impacts both refined and ozonized oils the various of the antibacterial impact at various time points. Anticancer role versus human prostate cancer cell line (PC-3) cells for oil forms was done using [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] MTT protocol. The change of cell cycles was compared in cancer cells and after treatment by examined oil forms. In vitro alpha glucosidase, alpha amylase and lipase enzymes inhibition was done in both oil samples relative to norms. Both oil forms contained 25 bioactive molecules where ozone exposure altered the molecular diversity with increased the number of classes of molecules in ozonized oil. Additionally, exposure to ozone…
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