Molecular, biofilm and motility characterization of Acinetobacter baumannii isolated from a neonatal intensive care unit at a children’s hospital in South China
Abstract
Background Multidrug-resistant Acinetobacter baumannii (MDR-AB) poses a critical threat in neonatal intensive care units (NICUs), however few studies have reported the molecular characterization of A. baumannii isolates from the NICU in China. This study aims to describe the molecular, biofilm and motility characteristics related to carbapenem resistance genes in A. baumannii isolates from the NICU. Methods Following an outbreak in March–April 2024, 21 A . baumannii isolates from neonatal and environmental sources were collected. These isolates underwent antimicrobial susceptibility testing, whole-genome sequencing (WGS) for resistance gene profiling and multi-locus sequence typing (MLST). Biofilm formation and twitching motility were measured in representative strains exposed to subinhibitory concentrations of gentamicin, levofloxacin, and meropenem. Results All 21 A . baumannii isolates were carbapenem-resistant Acinetobacter baumannii (CRAB), showing resistance to cephalosporins, aminoglycosides, and quinolones. WGS identified 25 resistance genes, including the universal bla OXA-23 , bla OXA-66 , bla ADC-25 and bla TEM-1D , aminoglycoside resistance genes ( armA , aph genes ), macrolide resistance genes ( mphE , msrE ), and tetracycline ( tetB ) resistance genes. Efflux pumps ( adeABC , adeFGH , adeIJK ) were ubiquitous. Two sequence types emerged: ST195 (20 isolates) and ST1959 (1 isolate), with high genetic similarity suggesting nosocomial transmission. ST1959 exhibited…
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