Effect of cyclodextrin complexes with chitosan and levofloxacin on the microflora of wounds and the colon of catfish
Abstract
The aim of the study is to investigate the effect of chitosan-β-cyclodextrin complexes with immobilized levofloxacin on some microbiological parameters of cut wounds and the large intestine of catfish. The experiment was conducted at the Progressive Biotechnologies in Aquaculture Research Laboratory of the N.I. Vavilov Saratov State University of Genetics, Biotechnology and Engineering. The course of the inflammatory process and the effectiveness of cyclodextrin-based treatments were characterized by determining the total microbial count of cut wounds, as well as the total microbial count and the number of lactic acid bacteria in the large intestine of juvenile African labyrinth catfish – Clarias gariepinus. Five groups of fish with cut wounds of the skin were formed (n = 10): 1st control, in which the fish received a regular balanced feed; 2nd control with a model of digestive disorders and intestinal dysbiosis; experimental groups 1, 2, and 3 received treatment with 20 %, 15, and 10 % levofloxacin, respectively. The fish received this treatment three times daily with their feed for 7 days. Wounds were created by making 2-cm incisions in the dorsal muscles near the dorsal fin. The wound surface was washed with sterile cotton swabs. The contents of the large intestine were extracted under sterile conditions after fish necropsy. The total microbial count (TMC) and lactic acid bacteria were determined by serial dilution and colony counting after culture.…
Read the paper
Related studies
- Ferulic acid regulates the AKT/GSK-3β/CRMP-2 signaling pathway in a middle cerebral artery occlusion animal model
- Novel Pharmacokinetic/Pharmacodynamic Parameters Quantify the Exposure–Effect Relationship of Levofloxacin against Fluoroquinolone-Resistant Escherichia coli
- Photochemical behavior of levofloxacin
- Correlation Between Dissolution Profiles of Salt-Form Drugs in Biorelevant Bicarbonate Buffer and Oral Drug Absorption: Importance of Dose/ Fluid Volume Ratio
- Evaluation of the synergistic potential and mechanisms of action for de novo designed cationic antimicrobial peptides
- Synthesis of [11C]levofloxacin
Go deeper in Evidence Hub
Evidence Hub adds a study data sheet (design, sample size, evidence level, key numbers), an AI summary and a chat grounded in the paper's text.