Microbiological study of wounds and large intestine of sturgeons using β-cyclodextrin complex with levofloxacin
Abstract
Background.The research laboratory Progressive biotechnologies in aquaculture of the Saratov State University of Genetics, Biotechnology and Engineering named after N.I. Vavilov studied the effect of complexes of ß-cyclodextrin on the healing of skin wounds in hybrid sturgeon fish. The total microbial count of sturgeon cut wounds was determined using the culture method and the composition of the microflora of the fish colon using the qPCR method under the influence of the ß-cyclodextrin complex. This complex was ß- cyclodextrin with levofloxacin included in a chitosan shell. The complex was adsorbed on the surface of the feed that the sturgeons received daily. Changes in the total microbial count demonstrate the nature of the inflammatory process and the effectiveness of the use of therapeutic drugs. The study of the colon microbiota also demonstrates the result of using these complexes in fish. It was found that the use of antibacterial complexes of ß-cyclodextrins with chitosan on sturgeons leads to a significant decrease in the total microbial count of the wound surface. The highest antimicrobial activity was established for the chitosan-ß-cyclodextrin complex with 20 and 15% levofloxacin content. The presence of bacteroides, eubacteria, clostridia, peptostreptocci, enterobacteria, lactobacilli and staphylococci were determined in the composition of the normal flora. At the same time, by the end of the experiment, the microbiome of the large intestine of fish is…
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