A highly sensitive terbium-keton complex-based fluorescent immunoassay for detection of methicillin-resistant Staphylococcus aureus (MRSA) antigen in milk, farmer hand swabs, and cattle drinking water
Abstract
This study presents the development and validation of a novel fluorescence-based immunoassay for the rapid and sensitive detection of methicillin-resistant Staphylococcus aureus (MRSA) antigen using a terbium-keton complex (Tb-2,keton) as the luminescent probe. The sensing platform operates through fluorescence enhancement upon specific interaction between the MRSA antigen and its corresponding antibody. Comprehensive spectroscopic characterization, including UV-vis absorption and fluorescence emission spectroscopy, was employed to investigate the optical properties and sensing mechanism. The Tb-2,keton complex exhibited characteristic terbium emission peaks, with the most intense signal observed at approximately 545 nm. Upon sequential addition of MRSA antigen and anti-MRSA antibody, significant fluorescence enhancement was observed, indicating successful immunocomplex formation. The biosensing system demonstrated concentration-dependent fluorescence responses, with the emission intensity increasing progressively with increasing antigen concentrations. The proposed method offers advantages of simplicity, rapid response (15 minutes), and high sensitivity for MRSA antigen detection. This work provides a foundation for developing terbium-based fluorescent immunoassays for pathogenic bacteria detection in clinical and food safety applications.
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Evidence Hub reads this as a experimental / empirical study, done in animal, not in people. Its evidence level is 5 of 5 (Very low · case / narrative / animal / in vitro / in silico). Case reports, narrative reviews, and animal, cell or computer-model studies give the weakest evidence for human health.
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