Quantitative Control of Zn2+ Photorelease: A Step toward Decoding Mechanisms of Subsecond Metal Signaling in the Brain
Abstract
Free ionic zinc (Zn2+) exerts control over many aspects of neuronal function after its exocytotic release from vesicles by associating with conserved binding sites on regulatory proteins, including the dopamine transporter. While the use of photoreactive cages to investigate the influence of subsecond Zn2+ transients on neurotransmitter release and uptake is useful, a quantitative understanding of Zn2+'s regulatory effects requires knowing how much Zn2+ has been released. Here, we have addressed this issue by directly measuring 4-methoxy-3-nitrobenzaldehyde (3-NPA), a photoreaction byproduct from [Zn(DPAeCageOMe)]+, as a reporter to quantify Zn2+ photorelease. We optimized a sawhorse waveform, which eliminated fouling and provided a linear oxidation current response as concentration was increased. We then demonstrated the utility of our method by measuring released zinc ions evoked by light exposure in whole brains harvested from adult zebrafish ex vivo. Our findings revealed that zinc, which was released at concentrations of about 1 to 4 μM in response to light application durations of 300 to 900 ms, significantly increased dopamine overflow and inhibited dopamine uptake. The profound effects of low concentrations of Zn2+ on release and uptake raise the possibility that Zn2+, which is released from terminals at concentrations of 10 to 20 μM, acts as a volume neuromodulator, with targets beyond respective postsynaptic terminals. Given that Zn2+ dysregulation is a…
Read the paper
Related studies
- Chaos and quantum thermalization
- The Dalton quantum chemistry program system
- Cord Blood Leptin and Adiponectin as Predictors of Adiposity in Children at 3 Years of Age: A Prospective Cohort Study
- Quantitative metabolomics by H-NMR and LC-MS/MS confirms altered metabolic pathways in diabetes
- Stable adiabatic quantum batteries
- A novel self-lipid antigen targets human T cells against CD1c(+) leukemias
Go deeper in Evidence Hub
Evidence Hub adds a study data sheet (design, sample size, evidence level, key numbers), an AI summary and a chat grounded in the paper's text.