Optimized and Cost-Effective Portable Electrochemical DNA Sensor for Dual-Gene Detection of Neisseria Gonorrhoeae in Patients Using In-House Printed Electrodes
Abstract
In this study, a screen-printed electrode modified with gold nanoparticle-stabilized graphene quantum dots and ssDNA probe (SPPE/AuNPs@GQDs/ssDNA) based biosensor compatible with smartphone technology for electrochemical detection of Neisseria gonorrhoeae (NG) via targeting two different genes ( FitA and MsrA ) was developed. The gold stabilized graphene quantum dots (AuNPs@GQDs) were synthesized and characterized using UV–vis spectroscopy, Fourier Transform Infrared Spectroscopy (FTIR), fluorometry, particle analyser, zeta potential and Transmission Electron Microscopy (TEM), confirming their successful synthesis and functionalization. The electrode surface characterization was assured through Scanning Electron Microscopy (SEM), Energy Dispersive X-ray (EDX) and FTIR. The electrochemical response of biosensor was investigated using cyclic voltammetry (CV), differential pulse voltammetry (DPV) and electrochemical impedance spectroscopy (EIS). The FitA and MsrA gene-based DNA sensor exhibited exceptional sensitivity, reaching 16,333.9 (μA/mm 2 )/ng, 140.38(μA/mm 2 )/ng with detection limits of 0.73 fg μl −1 , 100 fg μl −1 respectively via DPV. Selectivity and specificity studies revealed the sensor’s ability to differentiate NG DNA from non-target bacterial DNA and mismatched sequences, confirming its robustness. FitA and MsrA based validation studies using cervical swab samples further confirmed diagnostic potential and enabled cross-verification of enhanced specificity and…
Read the paper
Related studies
- Chaos and quantum thermalization
- The Dalton quantum chemistry program system
- Cord Blood Leptin and Adiponectin as Predictors of Adiposity in Children at 3 Years of Age: A Prospective Cohort Study
- Quantitative metabolomics by H-NMR and LC-MS/MS confirms altered metabolic pathways in diabetes
- Stable adiabatic quantum batteries
- A novel self-lipid antigen targets human T cells against CD1c(+) leukemias
Go deeper in Evidence Hub
Evidence Hub adds a study data sheet (design, sample size, evidence level, key numbers), an AI summary and a chat grounded in the paper's text.