Multiscale Imaging Reveals Compartment-Specific Mechanobiology of the Osteochondral Unit in Knee Osteoarthritis
Abstract
Mechanical overloading is a key risk factor for knee osteoarthritis (OA), yet its compartment-specific effects on the mechanobiology of the osteochondral unit and its interplay with synovial inflammation remain insufficiently defined. Here, multiscale imaging of clinical specimens is applied to uncover how mechanical and inflammatory cues shape the osteochondral unit in knee OA. Medial and lateral femoral condyles from patients with primary OA and secondary OA due to rheumatoid arthritis (RA), spanning varus to valgus alignment, were analyzed. A comprehensive multiscale imaging pipeline - including micro-computed tomography, cartilage- and bone-specific histomorphometry, quantitative backscattered electron imaging, and osteocyte lacunocanalicular network analysis - is integrated with targeted proteomic profiling of synovial fluid. Mechanical alignment is strongly associated with compartment-specific cartilage degeneration and subchondral bone adaptation in OA, as reflected by robust correlations between loading and OARSI score, subchondral bone thickness, bone formation, and osteocyte lacunocanalicular network parameters. Notably, the medial compartment exhibits substantially greater susceptibility to mechanically induced osteochondral remodeling than the lateral compartment. In contrast, these structure-function relationships are attenuated in RA. Collectively, these findings indicate that osteochondral remodeling in knee OA is predominantly associated with mechanical…
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