Determination of the Critical Voltage for the Observation of Uncoated Wood Samples in Electron Microscopy
Abstract
Electron microscopy (EM) is a key tool for studying the microstructure of wood; however, observing uncoated samples poses a challenge due to surface charging. This study aims to identify the critical voltage that allows for the effective observation of uncoated wood samples without significant loading. As part of the experiment, samples of different wood species were tested, including Acacia (Robinia pseudoacacia L.), Oak (Quercus robur L.), Maple (Acer pseudoplatanus spp.), Ash (Fraxinus excelsior L.), Spruce (Picea abies (L.) Karst.), Thermowood (Thermal modifed Spruce), Garapa (Apuleia leiocarpa), Ipé (Handroanthus spp.), Merbau (Intsia bijuga), and Massaranduba (Manilkara spp.). Several methods were tested for surface preparation for SEM analysis, including the use of a circular saw, a hand milling machine, and a microtome. The results show that the optimal voltage for observing uncoated wood samples varied depending on the wood species. Regarding the selection of wood species and the results obtained, it was found that uncoated samples could be effectively observed. This finding suggests that practical observations can be accelerated and more cost-effective, as all wood species exhibited the required voltage range of 1 kV to 1.6 kV. Additionally, it was determined that using a secondary electron detector was optimal for such observations, as it provided a sufficiently strong signal even at relatively low voltages. Conversely, when using a backscattered electron…
Read the paper
Related studies
- Characterization and Analysis of Porosity and Pore Structures
- Practical considerations for conducting ecotoxicity test methods with manufactured nanomaterials: what have we learnt so far?
- Dental Optical Coherence Tomography
- Radiation-shielding concrete: A review of materials, performance, and the impact of radiation on concrete properties
- Testing entanglement of annihilation photons
- Calanus finmarchicus seasonal cycle and diapause in relation to gene expression, physiology, and endogenous clocks
Go deeper in Evidence Hub
Evidence Hub adds a study data sheet (design, sample size, evidence level, key numbers), an AI summary and a chat grounded in the paper's text.