X-ray microtomography study of carious dentine and a comparison of its removal by three techniques
Abstract
The wisdom on mechanical removal of carious dentine based on hardness has been challenged and chemo-mechanical technique has been proposed as a more conservative method. However, the extent and comparison of excessive removal of sound dentine and insufficient removal of carious dentine have not been studied. The aims of the present study are to use X-ray microtomography (XMT) to determine the mineral concentrations of sound and carious dentine, and the excavated dentine using a hand excavation (HE) technique, a hand excavation technique aided by Caries Detector Dye (CD) and a chemo-mechanical removal technique using Carisolv (CSS). Comparison of the three techniques with respect to the mineral concentrations of the excavated dentine caries and the volumes of sound dentine removed were investigated. It was aimed to identify the boundary between ―infected‖ and ―affected‖ using the X-ray linear attenuation coefficients (LAC) from the XMT results and the ultrastructural images obtained from the Back Scattered Electron (BSE) imaging and Atomic Force Microscopy (AFM). Thirty eight deciduous molars with open carious cavities were sectioned in half. One half of each molar had the carious tissue removed by HE and the other by CSS or hand excavation plus CD. XMT images were taken before and after caries removal. From the data set, an assessor, who was ‗blind‘ to which technique was used, used LAC histograms to assess the efficacy of the excavation techniques. The volumes of sound…
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