Journey from light to quantum microscopy – special reference to advances in microscopy
Abstract
The journey from light to quantum microscopy has been an evolution of advancements. The journey commences with the revelation of light's nature and the development of basic microscopy. The augmented resolution of novel microscopes allow research into microorganisms and even the nano-level. The future of microscopy in exploring the microbiology seems propitious. Despite the adoption of various substitute methods for imaging live microorganisms, microbiologists still combat demur with modern light microscopy. Microscopy has become pivotal in medical sciences, particularly microbiology, and has bestowed to the development of micro and nanotechnologies. The earliest imaging technique, light microscopy, is extensively used in defiance of offering a low-resolution, 2D view of microorganisms. For more detailed imaging, electron microscopy—specifically scanning electron microscopy (SEM)— provides a clearer, more detailed view of microorganisms' morphology. Furthermore, confocal microscopy accredits the distinction of organelles in microorganisms through fluorescent marker staining, providing exhaustive and specific imaging of cellular structures. Holotomographic Microscopy (HTM), also known as Optical Diffraction Tomography (ODT), is a state-of-the-art technique that uses the interaction of light with different media within microorganisms. HTM offers a versatile substitute for cell imaging, overcoming many of the shortcoming of preceding techniques. Recent advances in microscopy…
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