Single Particle Tracking and Single Molecule Energy Transfer
Abstract
Preface PART I: Single-Particle Imaging and Tracking THREE DIMENSIONAL PARTICLE TRACKING IN A LASER SCANNING FLUORESCENCE MICROSCOPE Introduction Image-Based Single-Particle Tracking Methods Advanced Fluorescence Microscopy Techniques for Single-Particle Tracking Two-Photon Excitation Microscopy 3-D Tracking in Image-Based SPT Approaches 3-D Tracking in Laser Scanning Microscopes Instrumentation Background Noise Simultaneous Two-Particle Tracking Application: Chromatin Dynamics in Interphase Cells Conclusions THE TRACKING OF INDIVIDUAL MOLECULES IN CELLS AND TISSUES Introduction Single-Molecule and Single-Particle Localization Positional Accuracy Tracking Trajectory Analysis Applications Conclusions MESSENGER RNA TRAFFICKING IN LIVING CELLS Intranuclear Structure and Dynamics FCS and FRAP Studies of Nuclear mRNP Mobility Single-Particle Tracking of mRNA Molecules Single-Particle Tracking of Specific, Native mRNPs In Vivo Labeling of Native BR2 mRNPs Outlook: Light Sheet-Based Single-Molecule Microscopy QUANTUM DOTS: INORGANIC FLUORESCENT PROBES FOR SINGLE-MOLECULE TRACKING EXPERIMENTS IN LIVE CELLS Introduction Fluorescent Labels for Single-Molecule Tracking in Cells Optical Properties of Colloidal Quantum Dots Synthesis of Colloidal Fluorescent Quantum Dots Surface Chemistry for the Water-Solubilization of Quantum Dots Interfacing Quantum Dots with Biology Single Quantum Dot Tracking Experiments in Live Cells Conclusions and Perspectives PART II: Energy Transfer on the…
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