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A Reagentless Biosensing Assembly Based on Quantum Dot–Donor Förster Resonance Energy Transfer

Igor L. Medintz, Aaron R. Clapp, Joseph S. Melinger, Jeffrey R. Deschamps, Hedi M. Mattoussi

Advanced Materials, 2005

117 citations

Abstract

On the surface of a quantum dot (QD), maltose-binding protein (MBP, white and yellow in Figure) labeled with a cyanine dye (red) self-assembles, resulting in F?rster resonance energy transfer between the acceptor dye and the QD donor. Upon binding maltose, MBP undergoes a conformational change (white ⇌ yellow), causing concentration-dependent photoluminescence changes.

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