Genetic characteristics and antimicrobial resistance profile of non-typeable Haemophilus influenzae from the respiratory tract in a tertiary hospital in southwest China
Abstract
Abstract Background Non-typeable Haemophilus influenzae (NTHi) has emerged as the predominant cause of invasive Haemophilus influenzae (H. influenzae) disease. This microorganism exhibits extensive genomic heterogeneity, which complicates vaccine development and contributes to the evolution of antimicrobial resistance. Methods A total of 57 H. influenzae isolates collected from respiratory tract specimens at a tertiary hospital in southwest China between January and April 2025 were subjected to whole-genome sequencing on the Illumina platform. Clinical data from the corresponding patients and the antimicrobial susceptibility profiles of the isolates were collected for analysis. Results All 57 clinical isolates were identified as NTHi. Most isolates were recovered from male patients (75.4%) and adults aged ≥ 60 years (43.9%). Multilocus sequence typing (MLST) identified 41 distinct sequence types (STs), with ST422 being the most prevalent (7.0%, 4/57). A total of 54 virulence genes were conserved across all isolates and were predominantly associated with immune modulation. The highest resistance rate was observed for ampicillin (70.2%), followed by trimethoprim-sulfamethoxazole (54.4%) and ampicillin-sulbactam (47.4%). Resistance rates to amoxicillin-clavulanate and cefuroxime were 17.5% and 24.6%, respectively. All isolates remained susceptible to ceftazidime, ceftriaxone, cefepime, meropenem, and levofloxacin. β-Lactamase genes were detected in 49.1% of the isolates, and…
Read the paper
Related studies
- Ferulic acid regulates the AKT/GSK-3β/CRMP-2 signaling pathway in a middle cerebral artery occlusion animal model
- Novel Pharmacokinetic/Pharmacodynamic Parameters Quantify the Exposure–Effect Relationship of Levofloxacin against Fluoroquinolone-Resistant Escherichia coli
- Photochemical behavior of levofloxacin
- Correlation Between Dissolution Profiles of Salt-Form Drugs in Biorelevant Bicarbonate Buffer and Oral Drug Absorption: Importance of Dose/ Fluid Volume Ratio
- Evaluation of the synergistic potential and mechanisms of action for de novo designed cationic antimicrobial peptides
- Synthesis of [11C]levofloxacin
Go deeper in Evidence Hub
Evidence Hub adds a study data sheet (design, sample size, evidence level, key numbers), an AI summary and a chat grounded in the paper's text.