Microwave Assisted Acrylamide Grafting on a Natural Gum Cassia Tora: Characterization and Pharmacokinetic Evaluation of the Formulation Containing Metformin and Sitagliptin in Rats by LC-MS/MS
Abstract
Abstract A vast proportion of synthesis based polymers used to develop matrix drug delivery systems are too some extent harmful to patients who are taking those formulations for shorter and longer duration of time. Long term toxic effect of synthetic polymers can be countered by replacing those with substituted natural polymers. Under influence of microwave dielectric heating natural gums can be modified as promising biodegradable polymeric materials. The grafting as a tool to manipulate natural polysaccharides with the precise control over the features of the grafted polymer, under microwave irradiation can be a powerful strategy for the development of natural polymers with tailor made properties. The grafting of acrylamide onto cassia tora (CT) was carried out using microwave-assisted graft copolymerization and was characterized. The acute oral toxicity study in rodents was carried out according to OECD guideline. The pharmaceutical application in the tablet formulation of the acrylamide grafted CT (best batch) was studied by using metformin and sitagliptin as model drugs. It can be concluded that microwave assisted acrylamide grafting on cassia tora was successful. The developed formulation with the grafted CT as polymer was also studied in rats for pharmacokinetic profiling. The developed bioanalytical method to determine both the drugs in rat plasma was found simple, reproducible and validated as per the guidelines.
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