Effects of Gualou Xiebai Banxia Decoction on pulmonary vascular remodeling in rats with hypoxic pulmonary hypertension
Abstract
This study employed a combination of in vivo and in vitro experiments to investigate the effects and mechanism of Gualou Xiebai Banxia Decoction(GXBD) on pulmonary vascular remodeling in rats with hypoxic pulmonary hypertension(HPH). For the in vivo experiment, 36 specific-pathogen-free(SPF)-grade Sprague-Dawley(SD) rats were randomly divided into six groups(n=6 per group): control group, hypoxic model group, low-, middle-, and high-dose GXBD groups, and sildenafil group. The rats in the hypoxic model group and all treatment groups were exposed to a hypobaric oxygen chamber simulating an altitude of 5 500 m for 28 days to establish the HPH model, during which they received corresponding drug intervention, while the control group was raised normally. Relevant indicators were assessed using right ventricular catheterization, hematoxylin-eosin(HE) staining, transmission electron microscopy, and Western blot analysis. For the in vitro experiment, primary pulmonary artery smooth muscle cells(PASMCs) were isolated and cultured, and GXBD-containing serum was prepared. After exposure to 1% oxygen and screening of the optimal intervention concentration via cell counting kit-8(CCK-8), EdU staining, flow cytometry, and Western blot were used to detect cell proliferation, apoptosis, and the expression of related proteins. The results showed that compared to the hypoxic model group, all doses of GXBD significantly reduced the mean pulmonary artery pressure(mPAP) of rats, alleviated…
Read the paper
Related studies
- 2024 update in heart failure
- Vascular involvement in idiopathic pulmonary fibrosis
- A novel technique- subendometrial autologous platelet rich plasma injection in patients with unresponsive thin endometrium undergoing frozen-thawed embryo transfer: a prospective cohort study
- Does metformin prolong pregnancy in preterm pre-eclampsia? A study protocol for a South African, hospital-based double-blind, randomised, placebo-controlled trial
- Combined Pulmonary Fibrosis and Emphysema (CPFE): A "New" Smoking-Related Interstitial Lung Disease (ILD)
- Impact of an acute 1-month cannabidiol treatment on pain and inflammation after a long bone fracture: a triple-blind randomised, placebo-controlled, clinical trial protocol
Go deeper in Evidence Hub
Evidence Hub adds a study data sheet (design, sample size, evidence level, key numbers), an AI summary and a chat grounded in the paper's text.