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The relationships of trehalose and its metabolism to conidiation in Neurospora

D. L. Hanks, A. S. Sussman

1967

2 citations

Abstract

Relationship of trehalose to conidiation This spores is available in Fungal Genetics Reports: https://newprairiepress.org/fgr/vol11/iss1/12 Boylis, J. R., Jr. and A. G. DeBurk. Estimation of the frequency of multinucleate conidia in microconidiating rtroins. Frequency of multinucleate microconidia. Experiment Number I 2 3 ad-3 colonies x 104 per ml conidiol suspension 7.42 6 .40 76.00 lyr-3 colonies x 104 per ml conidial suspension 37.00 358.00 3 .8 Ratio od-3 colonies/ lyr-FJonies Tomable conidia x Iti l/5 l/56 20/l per ml conidiol suspension 44.42 364.40 79 .0 Wild type colonies per ml conidial suspension 8 3 5 3 19 Frequency @colonies 0.836 0.982 0.048 Frequency ad-3 colonies 0.166 0.017 0.952 Per cent wild +ype colonies 0.0187 c.0014 0.0024 Estimated per cent multin~cleate microconidia* 0.067 0.041 0.026 * This value is obtained from the following equation: %wild 2(~coIony frequency) (od-3 colony frequency). The frequency of multinucleate conicia in microconidioting strains of N. crosso was determined by means of a technique employing forced heterocoryonr. The strains used were of the following genotypes: *; pe,f1(38701; YB743m.L) and lyr-3; pe, f1(4545; YB743m, LT. Heterocoryons were formed by placing dropr of a mixed microconidiol suspension on plater of minimal medium. The heterocoryonr formed on the plates were transferred to minimal ogor slants and incubated. Micmconidiol suspensions from three independent heterocwyons were onolyzed. Each was filtered through Nitex…

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